Raman microscopy and surface‐enhanced Raman scattering (SERS) for in situ analysis of biofilms
Identifieur interne : 002690 ( Main/Exploration ); précédent : 002689; suivant : 002691Raman microscopy and surface‐enhanced Raman scattering (SERS) for in situ analysis of biofilms
Auteurs : Natalia P. Ivleva [Allemagne] ; Michael Wagner [Allemagne] ; Harald Horn [Allemagne] ; Reinhard Niessner [Allemagne] ; Christoph Haisch [Allemagne]Source :
- Journal of Biophotonics [ 1864-063X ] ; 2010-08.
English descriptors
- Teeft :
- Acquisition time, Aggregation agent, Analytical chemistry, Appropriate sers substrates, Biofilm, Biofilm analysis, Biofilm constituents, Biofilm matrices, Biofilm matrix, Biofilm samples, Biofilms, Biological samples, Chem, Chemical characterization, Chemical images, Clsm, Clsm image stacks, Colloid, Different structures, Enhancement, Gmbh, Ivleva, Kgaa, Laser, Laser spectroscopy, Matrix, Microbial, Microbial constituents, Microbiol, Multispecies, Munchen, Niessner, Nucleic acids, Online color, Optical microscope image, Polysaccharide, Raman, Raman images, Raman imaging, Raman microscopy, Raman shift, Raman spectra, Relative intensities, Reproducibility, Reproducible sers spectra, Sers, Sers analysis, Sers measurements, Sers spectra, Several orders, Spatial distribution, Spatial resolution, Technische universitat munchen, Various constituents, Verlag, Verlag gmbh, Water quality control, Weinheim, Weinheim journal.
Abstract
Biofilms are communities of micro‐organisms enclosed in a matrix of extracellular polymeric substances (EPS). They represent a ubiquitous form of microbial life on Earth. Detailed information on chemical composition and structure of the EPS matrix is relevant in medicine, industry and technological processes. Raman microscopy (RM) provides whole‐organism fingerprints for biological samples with spatial resolution in the μm range and enables correlations between optical and chemical images to be made. Low water background makes RM beneficial for in situ studies of biofilms, since water is the major component of the biofilm matrix. In this paper we discuss the feasibility of RM for chemical characterization of different structures in a multispecies biofilm matrix, including microbial constituents and EPS. We show that by improving the sensitivity of RM with surface‐enhanced Raman scattering (SERS) one can perform rapid biofilm analysis. In particular, by choosing appropriate SERS substrates and solving the problem of SERS measurement reproducibility one can carry out in situ study of different components in the complex biofilm matrix. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Url:
DOI: 10.1002/jbio.201000025
Affiliations:
- Allemagne
- Bavière, District de Haute-Bavière
- Garching bei München, Munich
- Université technique de Munich
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Le document en format XML
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<front><div type="abstract" xml:lang="en">Biofilms are communities of micro‐organisms enclosed in a matrix of extracellular polymeric substances (EPS). They represent a ubiquitous form of microbial life on Earth. Detailed information on chemical composition and structure of the EPS matrix is relevant in medicine, industry and technological processes. Raman microscopy (RM) provides whole‐organism fingerprints for biological samples with spatial resolution in the μm range and enables correlations between optical and chemical images to be made. Low water background makes RM beneficial for in situ studies of biofilms, since water is the major component of the biofilm matrix. In this paper we discuss the feasibility of RM for chemical characterization of different structures in a multispecies biofilm matrix, including microbial constituents and EPS. We show that by improving the sensitivity of RM with surface‐enhanced Raman scattering (SERS) one can perform rapid biofilm analysis. In particular, by choosing appropriate SERS substrates and solving the problem of SERS measurement reproducibility one can carry out in situ study of different components in the complex biofilm matrix. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)</div>
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